Enter the lyophilized mass stated on the vial and the volume of diluent being added. The calculator returns the resulting concentration and the mass contained in a given volume — the two numbers needed for accurate volumetric handling at the bench. Everything runs in your browser; nothing is sent anywhere.
1 · Lyophilized material in the vial
From the vial label or the certificate of analysis — not a guess.
2 · Diluent added
Bacteriostatic water or another diluent — this tool does not make that choice for you.
Mass contained by volume
How much material is present in a given volume of the solution above.
| Volume | Material contained |
|---|
Sterile, non-pyrogenic reconstitution solution with 0.9% benzyl alcohol preservative, USP grade — in 3 mL, 10 mL and 30 mL, shipped from the USA.
Reconstitution Solution →Concentration is mass divided by volume. A 10 mg vial reconstituted with 2 mL of diluent yields 5 mg/mL; the same vial with 1 mL yields 10 mg/mL. Nothing about the material changes — only how concentrated the resulting solution is, and therefore what volume corresponds to a given mass.
The volume displaced by the solid itself is very small at the masses typically supplied for research and is conventionally treated as negligible, so the final volume is taken as the volume of diluent added. Where that assumption matters, the final volume should be measured rather than assumed. Solubility and storage vary by compound — the lot Certificate of Analysis is the reference for the material in hand.
Lyophilized peptide is a freeze-dried solid. Reconstitution is the step of adding a measured volume of diluent so the material goes into solution at a known concentration.
Bacteriostatic water is common in laboratory settings because the benzyl alcohol preservative allows a prepared solution to be kept longer than one made with sterile water.
No. It reports the concentration of a solution and the mass contained in a stated volume, for laboratory reference. Products are for research use only.
Related: Certificates of Analysis